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aurum total rna 96 kit  (Bio-Rad)


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    Bio-Rad aurum total rna 96 kit
    Aurum Total Rna 96 Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aurum+total+rna+96+kit/Aurum+Total+RNA+96+Kit/pm41297592-120-13-19
    Average 93 stars, based on 19 article reviews
    aurum total rna 96 kit - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Extraction:

    Article Title: Responsiveness of PNPLA3 and lipid-related transcription factors is dependent upon fatty acid profile in primary bovine hepatocytes
    Article Snippet: .. As described therein, cells were harvested in TRIzol reagent (Invitrogen, Carlsbad, CA), RNA extracted using a phenol–chloroform extraction (Life Technologies), and RNA pooled and purified using the Aurum Total RNA 96 Kit (732–6800; Bio-Rad Laboratories, Hercules, CA). .. Quantified (Synergy H1 Hybrid Spectrophotometer; BioTek, Winooski, VT) and quality assured (ratio absorbances between 1.9 and 2.1 , ) RNA samples were reverse transcribed to cDNA using 0.5 μg RNA and iScript Reverse Transcription Supermix (170–8840; Bio-Rad Laboratories) in a C1000 Touch Thermo Cycler (Bio-Rad Laboratories).

    Article Title: Greater liver PNPLA3 protein abundance in vivo and in vitro supports lower triglyceride accumulation in dairy cows
    Article Snippet: .. Briefly, RNA was extracted via the manufacturer protocol (15596018; Life Technologies, ThermoFisher Scientific, Rockford, IL) using a phenol–chloroform extraction , purified, and subjected to on-column DNAse I (Bio-Rad Laboratories, Hercules, CA) digestion using the Aurum Total RNA 96 Kit (732-6800; Bio-Rad Laboratories) . .. Total RNA was eluted and then quantified using a Synergy H1 Hybrid Spectrophotometer (BioTek, Winooski, VT) as described , .

    Article Title: Mitochondrial function and nutrient partitioning in high and low feed efficient multiparous Holstein dairy cows.
    Article Snippet: .. Total RNA was extracted using a phenol, chloroform extraction and purified with the Aurum Total RNA 96 Kit (7326800, BioRad Laboratories, Hercules, CA) with modifications as previously described (Miller et al., 2010; Caputo Oliveira et al., 2020) and quantified using a Synergy H1 Hybrid Spectrophotometer (BioTek) with 260/280 absorbancesbetween 1.9 and 2.1; RNA was further assessed using a Bioanalyzer 2100 (Agilent Technologies Inc.) to obtain RNA integrity number for the total subset (mean ± SD: 6.95 ± 0.92). .. Total RNA (1 μg) was reverse transcribed into cDNA per the manufacturer protocol using iScript Reverse Transcription Supermix (1708840, Bio-Rad Laboratories) in a C1000 Touch Thermo Cycler (Bio-Rad Laboratories; Caputo Oliveira et al., 2020; Pralle et al., 2021).

    Article Title: Postpartum supplementation with fermented ammoniated condensed whey altered nutrient partitioning to support hepatic metabolism.
    Article Snippet: .. Total RNA was extracted from approximately 200 mg of liver stored in TRIzol, using a phenol, chloroform extraction (Chomczynski, 1993) and then purified utilizing the Aurum Total RNA 96 Kit (7326800; Bio-Rad Laboratories, Hercules, CA; Miller et al., 2011) through a modified version of the manufacturer’s protocol. ..

    Article Title: Liver and Muscle Transcriptomes Differ in Mid-Lactation Cows Divergent in Feed Efficiency in the Presence or Absence of Supplemental Rumen-Protected Choline.
    Article Snippet: .. Briefly, total RNA was extracted from ~200 g of tissue using a phenol, chloroform extraction [37] and then purified utilizing the Aurum Total RNA 96 Kit (7326800; Bio-Rad Laboratories, Hercules, CA, USA) with previously described modifications [36]. .. Total RNA was quantified using a Synergy H1 Hybrid Spectrophotometer (BioTek, Winooski, VT, USA) as described [36].

    Purification:

    Article Title: Responsiveness of PNPLA3 and lipid-related transcription factors is dependent upon fatty acid profile in primary bovine hepatocytes
    Article Snippet: .. As described therein, cells were harvested in TRIzol reagent (Invitrogen, Carlsbad, CA), RNA extracted using a phenol–chloroform extraction (Life Technologies), and RNA pooled and purified using the Aurum Total RNA 96 Kit (732–6800; Bio-Rad Laboratories, Hercules, CA). .. Quantified (Synergy H1 Hybrid Spectrophotometer; BioTek, Winooski, VT) and quality assured (ratio absorbances between 1.9 and 2.1 , ) RNA samples were reverse transcribed to cDNA using 0.5 μg RNA and iScript Reverse Transcription Supermix (170–8840; Bio-Rad Laboratories) in a C1000 Touch Thermo Cycler (Bio-Rad Laboratories).

    Article Title: Greater liver PNPLA3 protein abundance in vivo and in vitro supports lower triglyceride accumulation in dairy cows
    Article Snippet: .. Briefly, RNA was extracted via the manufacturer protocol (15596018; Life Technologies, ThermoFisher Scientific, Rockford, IL) using a phenol–chloroform extraction , purified, and subjected to on-column DNAse I (Bio-Rad Laboratories, Hercules, CA) digestion using the Aurum Total RNA 96 Kit (732-6800; Bio-Rad Laboratories) . .. Total RNA was eluted and then quantified using a Synergy H1 Hybrid Spectrophotometer (BioTek, Winooski, VT) as described , .

    Article Title: Hepatic pyruvate carboxylase expression differed prior to hyperketonemia onset in transition dairy cows
    Article Snippet: .. Hepatic RNA was extracted in TRIzol (Life Technologies) and purified utilizing the Aurum Total RNA 96 Kit (Bio-Rad Laboratories, Hercules, CA) using a modified version of the manufacturer’s protocol. .. Total RNA of samples was quantified and quality assured (ratio of absorbance at 260 and 280 nm between 1.9 and 2.1) using a Synergy H1 Hybrid Spectrophotometer (BioTek, Winooski, VT).

    Article Title: Mitochondrial function and nutrient partitioning in high and low feed efficient multiparous Holstein dairy cows.
    Article Snippet: .. Total RNA was extracted using a phenol, chloroform extraction and purified with the Aurum Total RNA 96 Kit (7326800, BioRad Laboratories, Hercules, CA) with modifications as previously described (Miller et al., 2010; Caputo Oliveira et al., 2020) and quantified using a Synergy H1 Hybrid Spectrophotometer (BioTek) with 260/280 absorbancesbetween 1.9 and 2.1; RNA was further assessed using a Bioanalyzer 2100 (Agilent Technologies Inc.) to obtain RNA integrity number for the total subset (mean ± SD: 6.95 ± 0.92). .. Total RNA (1 μg) was reverse transcribed into cDNA per the manufacturer protocol using iScript Reverse Transcription Supermix (1708840, Bio-Rad Laboratories) in a C1000 Touch Thermo Cycler (Bio-Rad Laboratories; Caputo Oliveira et al., 2020; Pralle et al., 2021).

    Article Title: Postpartum supplementation with fermented ammoniated condensed whey altered nutrient partitioning to support hepatic metabolism.
    Article Snippet: .. Total RNA was extracted from approximately 200 mg of liver stored in TRIzol, using a phenol, chloroform extraction (Chomczynski, 1993) and then purified utilizing the Aurum Total RNA 96 Kit (7326800; Bio-Rad Laboratories, Hercules, CA; Miller et al., 2011) through a modified version of the manufacturer’s protocol. ..

    Article Title: Liver and Muscle Transcriptomes Differ in Mid-Lactation Cows Divergent in Feed Efficiency in the Presence or Absence of Supplemental Rumen-Protected Choline.
    Article Snippet: .. Briefly, total RNA was extracted from ~200 g of tissue using a phenol, chloroform extraction [37] and then purified utilizing the Aurum Total RNA 96 Kit (7326800; Bio-Rad Laboratories, Hercules, CA, USA) with previously described modifications [36]. .. Total RNA was quantified using a Synergy H1 Hybrid Spectrophotometer (BioTek, Winooski, VT, USA) as described [36].

    Modification:

    Article Title: Hepatic pyruvate carboxylase expression differed prior to hyperketonemia onset in transition dairy cows
    Article Snippet: .. Hepatic RNA was extracted in TRIzol (Life Technologies) and purified utilizing the Aurum Total RNA 96 Kit (Bio-Rad Laboratories, Hercules, CA) using a modified version of the manufacturer’s protocol. .. Total RNA of samples was quantified and quality assured (ratio of absorbance at 260 and 280 nm between 1.9 and 2.1) using a Synergy H1 Hybrid Spectrophotometer (BioTek, Winooski, VT).

    Article Title: Postpartum supplementation with fermented ammoniated condensed whey altered nutrient partitioning to support hepatic metabolism.
    Article Snippet: .. Total RNA was extracted from approximately 200 mg of liver stored in TRIzol, using a phenol, chloroform extraction (Chomczynski, 1993) and then purified utilizing the Aurum Total RNA 96 Kit (7326800; Bio-Rad Laboratories, Hercules, CA; Miller et al., 2011) through a modified version of the manufacturer’s protocol. ..

    Spectrophotometry:

    Article Title: Mitochondrial function and nutrient partitioning in high and low feed efficient multiparous Holstein dairy cows.
    Article Snippet: .. Total RNA was extracted using a phenol, chloroform extraction and purified with the Aurum Total RNA 96 Kit (7326800, BioRad Laboratories, Hercules, CA) with modifications as previously described (Miller et al., 2010; Caputo Oliveira et al., 2020) and quantified using a Synergy H1 Hybrid Spectrophotometer (BioTek) with 260/280 absorbancesbetween 1.9 and 2.1; RNA was further assessed using a Bioanalyzer 2100 (Agilent Technologies Inc.) to obtain RNA integrity number for the total subset (mean ± SD: 6.95 ± 0.92). .. Total RNA (1 μg) was reverse transcribed into cDNA per the manufacturer protocol using iScript Reverse Transcription Supermix (1708840, Bio-Rad Laboratories) in a C1000 Touch Thermo Cycler (Bio-Rad Laboratories; Caputo Oliveira et al., 2020; Pralle et al., 2021).

    RNA Extraction:

    Article Title: Transcriptional Analysis of the Guinea Pig Mucosal Immune Response to Intravaginal Infection with Herpes Simplex Virus Type 2
    Article Snippet: Images were obtained using an EVOS XL Core Imaging system Life Technologies Corp., Carlsbad, CA). .. 4.3 RNA extraction and cDNA synthesis RNAs were extracted using the Aurum Total RNA 96 kit (Bio-Rad) following the manufacturer’s instructions and reverse-transcribed using the iScript cDNA Synthesis kit (Bio-Rad) as previously described ( Veselenak et al., 2015 ). ..

    cDNA Synthesis:

    Article Title: Transcriptional Analysis of the Guinea Pig Mucosal Immune Response to Intravaginal Infection with Herpes Simplex Virus Type 2
    Article Snippet: Images were obtained using an EVOS XL Core Imaging system Life Technologies Corp., Carlsbad, CA). .. 4.3 RNA extraction and cDNA synthesis RNAs were extracted using the Aurum Total RNA 96 kit (Bio-Rad) following the manufacturer’s instructions and reverse-transcribed using the iScript cDNA Synthesis kit (Bio-Rad) as previously described ( Veselenak et al., 2015 ). ..

    Article Title: Transcriptional Analysis of the Guinea Pig Mucosal Immune Response to Intravaginal Infection with Herpes Simplex Virus Type 2
    Article Snippet: Images were obtained using an EVOS XL Core Imaging system Life Technologies Corp., Carlsbad, CA). .. RNAs were extracted using the Aurum Total RNA 96 kit (Bio-Rad) following the manufacturer’s instructions and reverse-transcribed using the iScript cDNA Synthesis kit (Bio-Rad) as previously described ( Veselenak et al., 2015 ). ..

    Reverse Transcription:

    Article Title: Transcriptional Analysis of the Guinea Pig Mucosal Immune Response to Intravaginal Infection with Herpes Simplex Virus Type 2
    Article Snippet: Images were obtained using an EVOS XL Core Imaging system Life Technologies Corp., Carlsbad, CA). .. 4.3 RNA extraction and cDNA synthesis RNAs were extracted using the Aurum Total RNA 96 kit (Bio-Rad) following the manufacturer’s instructions and reverse-transcribed using the iScript cDNA Synthesis kit (Bio-Rad) as previously described ( Veselenak et al., 2015 ). ..

    Article Title: Transcriptional Analysis of the Guinea Pig Mucosal Immune Response to Intravaginal Infection with Herpes Simplex Virus Type 2
    Article Snippet: Images were obtained using an EVOS XL Core Imaging system Life Technologies Corp., Carlsbad, CA). .. RNAs were extracted using the Aurum Total RNA 96 kit (Bio-Rad) following the manufacturer’s instructions and reverse-transcribed using the iScript cDNA Synthesis kit (Bio-Rad) as previously described ( Veselenak et al., 2015 ). ..



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